Novel Use
AssigneeNuclera Nucleics Ltd.
InventorsMichael Chen, Jiahao Huang, Gordon McInroy
Life of this patent
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Abstract
The invention relates to the use of polyphosphate containing species in a method of nucleic acid synthesis, to methods of nucleic acid synthesis, and to the use of kits comprising said polyphosphate containing species. The invention also relates to the use of polyphosphate containing species for the capping of 3′-terminal hydroxyl moieties using terminal transferases.
Claims31
Use of a polyphosphate containing species as a permanent capping agent in a method of nucleic acid synthesis.
A method of nucleic acid synthesis, which comprises the steps of:
- a. providing an initiator sequence;
- b. adding a 3′-reversibly blocked nucleoside triphosphate to said initiator sequence in the presence of a terminal transferase or a functional equivalent or fragment thereof;
- c. adding a capping polyphosphate containing species;
- d. removal of all reagents from the initiator sequence;
- e. cleaving the blocking group from the 3′-reversibly blocked nucleoside triphosphate in the presence of a cleaving agent;
- and
- f. removal of the cleaving agent.
Use of a terminal transferase and a tripolyphosphate to phosphorylate the 3′-end of a nucleic acid species.
Dependencies are inferred from the claim text.