Sensors for detection of negatively charged phosphate-containing membranes and membrane components
AssigneeThe Governing Council of the University of Toronto
InventorsPatrick Thomas Gunning, Dzlyana Kraskouskaya, Aaron Cabral, Bronte Murcar-Evans, Krlmo Toutah, Elvln De Araujo
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Abstract
This application relates to a method of detecting negatively charged phosphate-containing membranes and membrane components, such as in cell membranes or artificial lipid vesicles, and its use, for example, in detecting apoptosis and bacterial infection.
Claims28
A method of detecting negatively charged phosphate-containing membrane components comprising:
- (a) combining a solution of a sample suspected of comprising negatively charged phosphate-containing membrane components with a solution comprising a compound of Formula I:
- wherein,
- W is a fluorophore;
- V is a linker moiety;
- Y is a metal ion coordinating moiety;
- M is a metal cation;
- and
- A is a weakly coordinating counter anion;
- and
- n is 1, 2 or 3;
- and
- (b) detecting a fluorescence signal at a wavelength specific for the fluorophore;
- wherein detection of the fluorescence signal in (b) indicates that the sample comprises negatively charged phosphate-containing membrane components.
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A compound of Formula IV:
- wherein:
- L1 is a linker group selected from C1-20alkylene, C2-20alkenylene, C2-20alkynylene and C3-10cycloalkylene, or a combination thereof, each of which is optionally oxo-substituted (═O) 1-3 times, and in which 1-3 carbon atoms are optionally replaced with a heteromoiety selected from NH, O, S and Si;
- and
- R2 is selected from
A compound of Formula V:
- wherein
- L2 is a linker group selected from C1-20alkylene, C2-20alkenylene, C2-20alkynylene and C3-10cycloalkylene, or a combination thereof, each of which is optionally oxo-substituted (═O) 1-3 times, and in which 1-3 carbon atoms are optionally replaced with a heteromoiety selected from NH, O, S and Si;
- and
- R3 is selected from
The compound of claim 47, wherein L2 is selected from:
The compound of claim 49, wherein the metal is Zn2+.
Dependencies are inferred from the claim text.