Non-Invasive Prenatal Test on Single Fetal Cells Isolated From Blood of Pregnant Women

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A method for isolating fetal cells from a maternal blood sample, comprising:

• supplying, by a polymer-coated laser microdissectible (PCLMD) chip, the maternal blood sample to an inlet of a microfluidic channel of a laser micro-dissectible (LMD) chip;
• and
• flowing, by the PCLMD chip, the maternal blood sample through the microfluidic channel and over a polymer membrane coating one or more surfaces of the microfluidic channel, the polymer membrane comprising an array of structures, wherein the array of structures comprises an array of pillar-like structures each having a diameter of 10 μm and each spaced apart from neighboring pillar-like structures by 10-15 μm, and wherein the array of pillar-like structures comprises an array of double-cross pillar structures, each double-cross pillar structure comprising two crosses intersecting at a center region of the double-cross pillar, each cross comprised of two arms of equal length intersecting at the center region, and wherein the double-cross pillar structures have similar size as the fetal cells and bind with the fetal cells to isolate fetal cells from a maternal blood sample.

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A method for isolating fetal cells from a maternal blood sample using a system for non-invasive prenatal testing, comprising a substrate coated with a cell-capturing surface, the cell-capturing surface including an array of pillar-like structures, each pillar-like structure including a plurality of intersecting arms, the method comprising the steps:

• supplying the maternal blood sample to an inlet of a microfluidic channel of a laser micro-dissectible (LMD) chip;
• and
• flowing the maternal blood sample through the microfluidic channel and over a polymer membrane coating one or more surfaces of the microfluidic channel, the polymer membrane comprising an array of structures configured to trap the fetal cells.