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US 2020/0269239 A1Published application
Fluid retention plates and analysis cartridges
AssigneeLuminex Corporation
InventorsRoss JOHNSON, Chuck COLLINS
Life of this patent
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Abstract
Fluid storage containers and analysis cartridges for use in assay processes are presented. In addition, systems comprising such storage containers and analysis cartridges and methods of using such containers and cartridges are presented as well. In specific embodiments, fluid storage containers are configured to be coupled to analysis cartridges in a first stage and a second stage.
Claims11
A method comprising:
- (a) adding a volume of a sample to a sample reservoir of a system, wherein the system comprises:
- (1) a first plate comprising:
- (i) a first side;
- (ii) a second side;
- (iii) a sample reservoir;
- (iv) a first port;
- (v) a channel connecting the sample reservoir and the first port;
- (vi) a sample dispensing channel opening onto the second side of the first plate, wherein the sample dispensing channel is in fluid communication with the sample reservoir;
- (2) a frangible blister coupled to the first side of the plate and covering the first port;
- (3) a second plate comprising:
- (i) a first side;
- (ii) a second side;
- (iii) a conduit passing through the second plate from the first side of the second plate to the second side of the second plate;
- wherein the first side of the second plate is coupled to the second side of the first plate;
- and
- (4) a printed circuit board retained on the second side of the second plate;
- wherein the printed circuit board comprises an electrowetting surface;
- (b) lysing the sample in the first plate, wherein the lysing comprises actuating the frangible blister to transfer a lysis buffer from the frangible blister to the sample reservoir via the first port and the channel connecting the sample reservoir and the first port;
- and
- (c) transferring the lysed sample from the first plate to the electrowetting surface of the printed circuit board via the conduit passing through the second plate.
17 more elements
Dependencies are inferred from the claim text.