Microfluidic device capable of removing microbubbles in channel by using porous thin film, sample injection device for preventing inflow of bubbles, and method for bonding panel of microfluidic element by using mold-releasing film

A sample injection device for preventing inflow of bubbles, the device comprising:

• a sample inlet into which a sample is injected;
• an upper panel formed with a microfluidic channel including a sample injection channel connecting a main channel to the sample inlet;
• a bubble inflow-preventing means formed in a portion of the microfluidic channel to be capable of holding bubbles included in the fluid;
• a porous thin film attached to a bottom surface of the microfluidic channel to remove bubbles included in the fluid passing through the microfluidic channel;
• a lower panel contacting the upper panel and a bottom surface of the porous thin film and having a path for discharging bubbles, which pass through the porous thin film, to the outside;
• and
• a vacuum-suctioning means for vacuum-suctioning the upper panel and the lower panel such that the microfluidic channel, to which the porous thin film is attached, is attached to the lower panel in a vacuum state.

A method for bonding panel of a microfluidic element by using a releasing film, the method comprising:

• a) preparing a panel having a pattern for a microfluidic channel formed on one surface thereof;
• b) preparing a release film coated with silicon nanoparticles on one or both surfaces thereof;
• c) plasma-treating one surface of the panel on which the pattern for the microfluidic channel is formed and the surface of the release film;
• and
• d) bonding the panel and the release film by performing heat treatment at a predetermined temperature range in a state in which the plasma-treated panel and the release film are attached to each other.