Liquid platelet-rich fibrin as a carrier system for biomaterials and biomolecules
OwnerRichard J. MIRON, Yufeng ZHANGinventors
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Abstract
Methods are provided for preparing liquid PRF and using the liquid PRF as a drug-delivery carrier system for other regenerative biomaterials and biomolecules. This carrier system provides more effective regenerative strategies for improved tissue wound healing, treatment, and regeneration in the body by supplying additional autologous growth factors into commonly-utilized biomaterials improving the host-tissue response to such therapies.
Claims22
A method of preparing an isolated serum fraction of liquid platelet-rich fibrin (PRF) comprising:
- spinning a whole blood sample by centrifugation to separate erythrocytes;
- separating a sample of liquid platelet-rich fibrin (PRF) from the whole blood sample without the addition of an additive;
- immediately spinning the sample of liquid PRF by centrifugation carried out at 20 to 950 G for 2 to 8 minutes;
- and
- prior to formation of a fibrin matrix of PRF, collecting an isolated serum fraction containing the liquid PRF using an application device.
A composition comprising an isolated serum fraction of liquid platelet-rich fibrin (PRF) and at least one regenerative biomaterial, the composition being obtained by a method comprising:
- spinning a whole blood sample by centrifugation to separate erythrocytes;
- separating a sample of liquid PRF from the whole blood sample without the addition of an additive;
- immediately spinning the sample of liquid PRF by centrifugation carried out at 20 to 950 G for 2 to 8 minutes;
- collecting the isolated serum fraction containing the liquid PRF using an application device;
- and
- prior to formation of a fibrin matrix of PRF, combining the isolated serum fraction containing the liquid PRF with the regenerative biomaterial.
A method of treating an injured tissue in an individual comprising:
- determining a site of tissue injury in the individual;
- administering, using an injectable application device, an isolated serum fraction of liquid platelet-rich fibrin (PRF) into and around the site of tissue injury;
- and
- administering, using an injectable application device, at least one regenerative biomaterial into and around the site of tissue injury.
Dependencies are inferred from the claim text.