Nucleic acid constructs and methods of use
AssigneesIntellia Therapeutics, Inc., Regeneron Pharmaceuticals, Inc.
InventorsJohn Finn, Hon-Ren Huang
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Abstract
Nucleic acid constructs that allow insertion and/or expression of a sequence of interest, such as a transgene, are provided. Compositions and methods of using such constructs for expression of a polypeptide or therapeutic agent, for example, are also provided.
Claims42
A bidirectional nucleic acid construct comprising:
- a) a first segment comprising a coding sequence for an agent;
- and
- b) a second segment comprising a reverse complement of a coding sequence of the agent, wherein the construct does not comprise a promoter that drives the expression of the agent.
A bidirectional nucleic acid construct comprising:
- a) a first segment comprising a coding sequence for a first agent;
- and
- b) a second segment comprising a reverse complement of a coding sequence of a second agent,
- wherein the construct does not comprise a promoter that drives the expression of the agents(s).
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A viral vector comprising a self-complementary (or double-stranded) nucleic acid construct that comprises a nucleotide sequence encoding a polypeptide, wherein the vector does not comprise a promoter that drives the expression of the polypeptide.
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Dependencies are inferred from the claim text.