Rna containing compositions and methods of their use
AssigneesIcahn School of Medicine at Mount Sinai, The Institute For Advanced Study - Louis Bamberger and Mrs. Felix Fuld Foundation, Ecole Normale Superieure, Centre National De La Recherche Scientifique, Universite Paris Diderot, Sorbonne Universite
InventorsBenjamin D. Greenbaum, Nina Bhardwaj, Arnold Levine, Remi Monasson, Simona Cocco
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Abstract
The present invention relates to a composition comprising an isolated, single stranded RNA molecule having a nucleotide sequence comprising 20 or more bases and a pattern of CpG dinucleotides defined by a strength of statistical bias greater than or equal to zero, and a pharmaceutically acceptable carrier suitable for injection. The present invention also relates to a kit comprising a cancer vaccine and the composition of the present invention as an adjuvant to the cancer vaccine. The present invention further relates to a method of treating a subject for a tumor and a method of stimulating an immune response.
Claims9
A method of making a composition comprising:
- isolating a single stranded RNA molecule of mammalian origin having a nucleotide sequence comprising 20 or more bases and a pattern of CpG dinucleotides defined by a strength of statistical bias greater than or equal to zero,
- wherein the strength of the statistical bias for CpG dinucleotides is determined for an RNA molecule that has a nucleotide sequence (x(S0)) by maximizing the probability of a sequence (S0) over x, where
- P
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- (
- S
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- Z
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- f
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- [
- EQUATION
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- 1
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- Z
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- x
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- sequences
-
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- [
- EQUATION
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- 2
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- Zm(x) is the normalization constant,
- P(S|x,m) is the probability of the sequence given the force (x) and motif m,
- x is the force on the motif that introduces a statistical bias over P,
- Nm(S) is the number of observed motifs, and
- f0(s) is the number of observed motifs,
- modifying at least one nucleic acid in the isolated RNA molecule, and
- including a pharmaceutically acceptable carrier suitable for injection.
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9-23. (canceled)
Dependencies are inferred from the claim text.