Rodents having genetically modified sodium channels and methods of use thereof
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsNicole Alessandri-Haber, Andrew J. Murphy, Lynn Macdonald
Life of this patent
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Abstract
Disclosed herein are rodents (such as mice and rats) genetically modified at an endogenous Scn9a locus to comprise an exogenous Scn nucleotide sequence such as the coding sequence of a human SCN2A gene. Also disclosed are methods and compositions useful for making such rodents, and methods of using such rodents for generating anti-NaV1.7 antibodies.
Claims37
A genetically modified rodent whose genome comprises:
- a nucleic acid molecule encoding a NaV1.2 protein at an endogenous rodent Scn9a locus,
- wherein the NaV1.2 protein is expressed in the genetically modified rodent, and
- wherein the rodent is a mouse or a rat.
A method of making a genetically modified rodent, comprising:
- modifying a rodent genome such that the modified genome comprises a nucleic acid molecule encoding a NaV1.2 protein at an endogenous rodent Scn9a locus, and
- generating a rodent comprising the modified genome, wherein the NaV1.2 protein is expressed in the genetically modified rodent, and wherein the rodent is a mouse or a rat.
A method of making a genetically modified rodent, comprising:
- (i) introducing a nucleic acid molecule encoding a NaV1.2 protein into a rodent embryonic stem (ES) cell such that the nucleic acid molecule integrates into an endogenous rodent Scn9a locus;
- (ii) obtaining a rodent ES cell comprising a modified genome, wherein the nucleic acid molecule has integrated into an endogenous rodent Scn9a locus;
- and
- (iii) generating a rodent using the rodent ES cell comprising the modified genome.
-46. (canceled)
An isolated rodent cell or tissue, whose genome comprises:
- a nucleic acid molecule encoding a NaV1.2 protein at an endogenous rodent Scn9a locus,
- wherein the rodent is a mouse or a rat.
-50. (canceled)
(canceled)
-58. (canceled)
A hybridoma that produces an anti-human NaV1.7 antibody.
(canceled)
Dependencies are inferred from the claim text.