Anti- isoasp7 amyloid ß (aß) antibodies and uses thereof

A method of determining the percentage of Aβ peptide comprising L-isoAsp at position 7 of SEQ ID NO:

• 1 in an isolated sample comprising:
• (a) quantifying the amount of L-isoAsp-comprising peptide through the use of a sandwich immunoassay, wherein the immobilized capture antibody or antigen-binding fragment thereof specifically binds to L-isoAsp7 Aβ, and the detection antibody is an antibody or antigen-binding fragment thereof that specifically binds to a polypeptide comprising SEQ ID NO:
• 1;
• (b) quantifying the total amount of Aβ through the use of a sandwich immunoassay, wherein the immobilized capture antibody or antigen-binding fragment thereof is an antibody or antigen-binding fragment thereof that specifically binds to a polypeptide comprising SEQ ID NO:
• 1, and the detection antibody is an antibody or antigen-binding fragment thereof that specifically binds to a polypeptide comprising SEQ ID NO:
• 1;
• and
• (c) determining the percentage value by using the values obtained in steps (a) and (b),
• wherein the KD of the interaction between the antibody that specifically binds to L-isoAsp7 Aβ and SEQ ID NO:
• 44 is at least 10 times less than the KD of the interaction between the same antibody and SEQ ID NO:
• 8 and the KD is determined by surface plasmon resonance at 25° C.