Detection of Fumarate Ester-Derived Modification in a Test Sample
AssigneeUniversity of South Carolina
InventorNORMA FRIZZELL CATLEDGE
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Abstract
Examination of a test sample to determine the presence or quantity of succination of proteins is described. Examination can be via protein hydrolysis in total succination determination or via enzymatic digestion of isolated proteins and determination of the presence or quantity of modified peptides. The methods can be utilized for determination of excessive succination of lymph system proteins, which can be utilized in prevention or early detection of lymphopenia. Methods can be utilized for test samples of subjects under treatment with dimethyl fumarate suffering from multiple sclerosis. Methods can be utilized as a determination that treatment of the subject with DMF should be slowed or stopped.
Claims20
A method for determining a succination level in a test sample, comprising:
- hydrolyzing proteins of a test sample;
- determining the presence or quantity of modified cysteines in the hydrolyzed proteins, wherein the modified cysteines have been modified by one or more of dimethyl fumarate, monomethyl fumarate, or fumarate.
A method for determining the succination level in a test sample, the method comprising:
- isolating peripheral blood mononuclear cells of the sample;
- lysing the peripheral blood mononuclear cells;
- enzymatically digesting proteins of the lysed peripheral blood mononuclear cells;
- and
- determining the presence or quantity of one or more modified peptides in digested proteins, the one or more modified peptides including at least one cysteine modified with dimethyl fumarate, monomethyl fumarate, or fumarate.
Dependencies are inferred from the claim text.