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AssigneeGE Healthcare Bio-Sciences AB
InventorsAsa Frostell, Elisabeth Wallby, Asa Hagner-McWhirter, Linnea Nygren Babol
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The present invention relates to a method for virus assay. More closely the invention relates a method for total quantification of adenovirus in a sample as well as total and functional (active) adenovirus in a sample. The method for determining adenovirus concentration in a sample comprises subjecting said sample to SPR (surface plasmon resonance) assay with immobilized FX (Factor X) and/or immobilized CAR (coxsackievirus and adenovirus receptor) on a sensor surface, wherein the adenovirus concentration is determined from sample binding to immobilized FX and/or immobilized CAR. CAR can be replaced by an ligand binding to adenovirus fiber, such as an anti-adenovirus fiber antibody. FX can be replaced by a ligand binding to adenovirus hexon, such as an anti-adenovirus hexon antibody. The method can be used for quality control in an adenovirus purification process, for example for gene therapy.
A method for determining adenovirus concentration in a sample, comprising subjecting said sample to surface plasmon resonance (SPR) assay with immobilized Factor X (FX) and/or immobilized coxsackievirus and adenovirus receptor (CAR) on a sensor surface, wherein the adenovirus concentration is determined from sample binding to immobilized FX and/or immobilized CAR.
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