Diagnosis of systemic lupus erythematosus using oligonucleotides antigens
AssigneeYeda Research and Development Co. Ltd.
InventorsIrun R. COHEN, Eytan DOMANY, Noam SHENTAL, Ittai FATTAL
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Abstract
Methods and kits for diagnosing systemic lupus erythematosus (SLE) in a subject are provided. Particularly, the present invention relates to specific oligonucleotide antibody reactivities useful in diagnosing SLE in a subject.
Claims15
A method of determining the reactivity of antibodies in a sample of a subject suspected of having SLE to a plurality of antigens comprising at least one oligonucleotide antigen selected from the group consisting of SEQ ID NOs:
- 1, 3, 5, 7 22, 28 and 42, and at least one additional oligonucleotide antigen selected from the group consisting of SEQ ID NOs:
- 8, 10, 17, 18, 28, 34, 36, 38, 42, 43, 44, 65, 66 and 67, the method comprising:
- i. obtaining a serum, plasma sample or blood sample from the subject;
- ii. providing the plurality of antigens in the form of an antigen probe set, an antigen array, or an antigen chip,
- iii. contacting the sample, under conditions such that a specific antigen-antibody complex may be formed, with said plurality of antigens, and
- iv. quantifying the amount of antigen-antibody complex formed for each antigen, by a system that allows quantitative measurement of antigen-antibody binding.
A method of determining the reactivity of antibodies in a sample of a subject suspected of having SLE to a plurality of antigens comprising at least two oligonucleotide antigens selected from the group consisting of SEQ ID NOs:
- 42, 5, 28, 1, 3, 7 and 22, the method comprising:
- i. obtaining a sample from the subject;
- ii. contacting the sample, under conditions such that a specific antigen-antibody complex may be formed, with said plurality of antigens, and
- iii. quantifying the amount of antigen-antibody complex formed for each antigen.
Dependencies are inferred from the claim text.