Method for acquiring information on analyte

A method for acquiring information on an analyte, comprising the steps of:

• bringing a polypeptide that is the analyte into contact with a first capture substance that binds to the polypeptide and a second capture substance that binds to the polypeptide to form a complex comprising the polypeptide, the first capture substance and the second capture substance;
• bonding the first capture substance in the complex to a first solid phase to immobilize the complex onto the first solid phase;
• collecting the first solid phase on which the complex immobilized;
• releasing the complex from the collected first solid phase, and then bonding the second capture substance in the released complex to a second solid phase to immobilize the complex onto the second solid phase;
• and
• acquiring information on the polypeptide from the complex immobilized on the second solid phase,
• wherein the first capture substance and/or the second capture substance binds to a C-terminal region of the polypeptide.

A method for acquiring information on an analyte, comprising the step of acquiring information of a polypeptide that is the analyte from a complex comprising the polypeptide and a capture substance that binds to the polypeptide,

• wherein
• the complex is produced by:
• contacting the polypeptide with the capture substance to form a complex comprising the polypeptide and the capture substance;
• bonding the capture substance in the complex to a first solid phase to immobilize the complex onto the first solid phase;
• collecting the first solid phase comprising the complex immobilized on the first solid phase;
• and releasing the complex from the collected first solid phase, and then bonding the capture substance in the released complex to a second solid phase,
• wherein the capture substance binds to a C-terminal region of the polypeptide.