Cell-based assay for quantifying the potency and efficacy of cannabinoids and/or terpenoids, and methods of use thereof
AssigneeCannaMetrix, LLC
InventorHarold C. SMITH
Life of this patent
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Abstract
The present disclosure relates to, inter alia, cell-based assays, recombinant cell lines, and methods for quantifying the potency and efficacy of cannabinoids and/or terpenoids.
Claims33
A cell-based assay for quantifying the potency and/or efficacy of one or more cannabinoids and/or terpenoids, alone or in combination using cellular mechanism as a report, said assay comprising a reaction template for contacting a cannabinod or terpenoid containing sample with a reagent and a means for measuring any biological response.
A human cell line for use in quantifying the potency and/or efficacy of one or more cannabinoid and/or terpenoid, alone or in combination.
A method for preparing a human cell line for use in quantifying the potency and/or efficacy of one or more cannabinoid and/or terpenoid, alone or in combination, said method comprising steps of engineering human cells to express either human CB1 or CB2, or chimeric proteins thereof. and a promiscuous recombinant human beta/gamma subunit that enable the otherwise suppression of calcium release by CB receptors to be a stimulation of calcium release due to cannabinoid signaling
Dependencies are inferred from the claim text.