Detection of mycoplasma in cell cultures and cell culture derived biologicals
AssigneeAkron Biotechnology, LLC
InventorsClaudia Zylberberg, Juan Manuel Rodriguez, Andres Clemente Hernando Insua, Ricardo Ariel Lopez
Life of this patent
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Abstract
Sequences having specificity for Mycoplasma and related Mollicutes genus strains and uses thereof. Methods of use include detection of samples contaminated with Mycoplasma. Kits are provided and comprise one or more oligonucleotides for the detection of Mycoplasma and related Mollicutes genus strains.
Claims25
An isolated or synthetic nucleic acid, wherein the isolated or synthetic nucleic acid hybridizes to at least 5 consecutive nucleic acids in a 5′ to 3′ or 3′ to 5′ direction of a 16S-23S ribosomal intergenic region nucleic acid of Mycoplasma and/or related Mollicutes genus strains.
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A nucleic acid sequence, wherein the nucleic acid sequence is complementary to at least five consecutive nucleic acids in a 5′ to 3′ or 3′ to 5′ direction, of any one or more sequences set forth as SEQ ID NOS:
- 1 to 15.
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A nucleic acid sequence set forth as SEQ ID NOS:
- 1 to 15 or fragments thereof, comprising one or more nucleic acid base mutant, variants, analogs, deletions, substitutions or combinations thereof.
A method of detecting whether a sample is contaminated with Mycoplasma and/or related Mollicutes genus strains comprising:
- incubating a sample with one or more primers or oligomers comprising nucleic acid sequences set forth as SEQ ID NOS:
- 1 to 15;
- assaying the samples;
- thereby,
- detecting whether the sample is contaminated with Mycoplasma and/or related Mollicutes genus strains.
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Dependencies are inferred from the claim text.