Methods and kits for targeted enrichment of target dna with high gc content
AssigneeBerry Genomics Co., Ltd.
InventorsZiwei QU, Tao YU, Fangming WANG, Nannan ZHANG, Jianguang ZHANG
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Abstract
The present invention relates to a method for enrichment of target DNA with high GC content based on target sequence capture and multiple displacement amplification, as well as a kit suitable for this method. The present invention also relates to a method for constructing a sequencing library of target DNA with high GC content based on the enrichment method of the present invention.
Claims16
A method for enrichment of target DNA with high GC content, comprising the following steps:
- (a) designing single-stranded oligonucleotide probes against specific flanking sequences of the target DNA with high GC content, and capturing the target DNA from genome DNA by target sequence capture technology;
- (b) amplifying the captured target DNA with high GC content using multiple displacement amplification so as to obtain amplification product;
- (c) subjecting the amplification product to enzyme digestion to remove branched DNA intermediates, so as to obtain digested product;
- (d) purifying DNA fragment with a length equal to or larger than 2 kb, so as to obtain enriched target DNA with high GC content.
A kit for enrichment of target DNA with high GC content, comprising reagents for capturing target DNA with high GC content, reagents for multiple displacement amplification, reagents for removing branched DNA intermediates, and reagents for purifying DNA fragments.
Dependencies are inferred from the claim text.