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AssigneeKikkoman Corporation
InventorsYosuke MASAKARI, Atsushi ICHIYANAGI
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Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus Coniochaeta and represented by SEQ ID No: 1 or 3, and having residual activity even in the presence of a surfactant. The present invention also provides a composition and kit for use in measuring glycated hemoglobin, comprising a specific stabilizer and/or a buffer. The present invention can provide an enzyme and a composition for use in measuring glycated hemoglobin, excellent in storage stability even if they are exposed to a surfactant.
A method for measuring glycated hemoglobin said method comprising use of one or more surfactants and an amadoriase, wherein the amadoriase comprises an amino acid sequence having an identity of at least 90% with the amino acid sequence as shown in SEQ ID NO:
A method for maintaining the residual activity of an amadoriase contained in reagents for measuring glycated hemoglobin in the presence of an ionic surfactant or for alleviating reduction of the residual activity of an amadoriase contained in reagents for measuring glycated hemoglobin in the presence of an ionic surfactant, said method comprising using one or more ionic surfactant, an amadoriase, and a stabilizer that is capable of maintaining the residual activity of an amadoriase in the presence of an ionic surfactant or capable of alleviating reduction of the residual activity of an amadoriase in the presence of an ionic surfactant, wherein said stabilizer is selected from the group consisting of phosphoric acid, a tricarboxylic acid, a dicarboxylic acid, a monocarboxylic acid, a compound represented by the formula (IV)
A method for maintaining the residual activity of an amadoriase contained in reagents for measuring glycated hemoglobin in the presence of an ionic surfactant or for alleviating reduction of the residual activity of an amadoriase contained in reagents for measuring glycated hemoglobin in the presence of an ionic surfactant, said method comprising using one or more ionic surfactant, an amadoriase, and a buffer that is capable of maintaining the residual activity of an amadoriase in the presence of an ionic surfactant or is capable of alleviating reduction of the residual activity of an amadoriase in the presence of an ionic surfactant, wherein said buffer is selected from the group consisting of a borate buffer, a Tris-HCl buffer, a phosphate buffer, a citrate buffer, a fumarate buffer, a glutarate buffer, a citraconate buffer, a mesaconate buffer, a malonate buffer, a tartrate buffer, a succinate buffer, an adipate buffer, ACES (N-(2-acetamido)-2-aminoethanesulfonic acid) buffer, BES (N,N-bis(2-hydroxyethyl)-2-amino-ethanesulfonic acid) buffer, Bicin (N,N-bis(2-hydroxyethyl)glycine) buffer, Bis-Tris (bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane) buffer, EPPS (4-(2-hydroxyethyl)-1-piperazine-propanesulfonic acid) buffer, HEPPSO (N-(hydroxyethyl)piperazine-N′-2-hydroxypropanesulfonic acid) buffer, MES (2-(n-morpholino)ethanesulfonic acid) buffer, MOPS (3-(N-morpholino)propanesulfonic acid) buffer, MOPSO (2-hydroxy-3-morpholino-propanesulfonate) buffer, PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid)) buffer, POPSO (piperazine-1,4-bis(2-hydroxypropanesulfonic acid)) buffer, TAPS (N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid) buffer, TAPSO (3-[N-tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid) buffer, TES (N-tris(hydroxymethyl)methyl-2-amino-ethanesulfonic acid) buffer, Tricine (N-Tris(hydroxymethyl)methylglycine) buffer and a combination thereof.
Dependencies are inferred from the claim text.