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OwnerFlorian M. Wurm, Maria J. Wurm +6inventors
InventorsFlorian M. Wurm, Maria J. Wurm, Maria de Lourdes Rodrigues, Divor Kiseljak, Cedric Bürki, Cyril Pugin, Guillaume Raussin, Julie Heymoz
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The present invention provides robust, streamlined, reproducible and highly efficient eukaryotic cell transfection systems and related methods. The highly-efficient systems and methods of the present invention reduce the number of steps required to transfect cells and reduce, e.g., eliminate, the need for specialized equipment. In particular, the systems and related methods afford the ability for streamlining transfection while retaining and improving robust and reproducible transfection efficiencies, cell viability, and/or protein production. Furthermore, the highly-efficient systems and methods of the present invention for transfecting eukaryotic cells also eliminate the need for any specialized or complicated preparation of exogenous nucleic acid, which makes available high throughput and/or large scale transfection.
A highly-efficient eukaryotic cell transfection (HECT) system that is capable of mediating transfection of purified, unencumbered exogenous nucleic acid into a eukaryotic cell comprising a cell transfection medium pre-conditioned with a positively charged polymer.
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Dependencies are inferred from the claim text.