Genomic insulator elements and uses thereof
AssigneeUniversity of Washington
InventorsGeorge Stamatoyannopoulos, John Stamatoyannopoulos
Life of this patent
Estimating the term…
Abstract
Provided herein are methods for identifying high potency genomic insulator elements that can be used in a vector composition e.g., that are useful for preventing unwanted expression of neighboring genes, such as proto-oncogenes, when administered to a subject in need thereof. Also provided herein are methods for treating disease and methods for administering a nucleic acid to a subject using such vectors.
Claims21
-51. (canceled)
An expression cassette comprising a nucleic acid sequence encoding a therapeutic agent driven by a promoter regulated by an enhancer,
- wherein the cassette is bracketed by genomic insulator elements,
- wherein each of the genomic insulator elements comprises a CTCF binding site core sequence consisting of the nucleotide sequence set forth in SEQ ID NO:1.
An expression cassette comprising a nucleic acid sequence encoding a therapeutic agent driven by a promoter regulated by an enhancer,
- wherein the cassette is bracketed by genomic insulator elements,
- wherein each of the genomic insulator elements comprises a CTCF binding site core sequence consisting of the nucleotide sequence set forth in one of SEQ ID NOs:
- 2-6.
Dependencies are inferred from the claim text.