Simian Adenoviruses SAdV-43, -45, -46, -47, -48, -49, and -50, and Uses Thereof
AssigneeThe Trustees of the University of Pennsylvania
InventorsSoumitra Roy, James M. Wilson, Luc H. Vandenberghe
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Abstract
A recombinant vector comprises simian adenovirus 43, 45, 46, 47, 48, 49 or 50 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus 43, 45, 46, 47, 48, 49 or 50 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
Claims17
A recombinant adenovirus having an adenovirus capsid comprising a capsid protein selected from the group consisting of:
- (a) a hexon protein of SAdV-45, amino acids 1 to 953 of SEQ ID NO:
- 48;
- a hexon protein of SAdV-47, amino acids 1 to 958 of SEQ ID NO:
- 109;
- a hexon protein of SAdV-48, amino acids 1 to 934 of SEQ ID NO:
- 139;
- a hexon protein of SAdV-49, amino acids 1 to 916 of SEQ ID NO:
- 167;
- a hexon protein of SAdV-50, amino acids 1 to 920 of SEQ ID NO:
- 193;
- (b) a penton protein of SAdV-45, amino acids 1 to 664 of SEQ ID NO:
- 43;
- a penton protein of SAdV-46, amino acids 1 to 563 of SEQ ID NO:
- 74;
- a penton protein of SAdV-43, amino acids 1 to 651 of SEQ ID NO:
- 6;
- a penton protein of SAdV-47, amino acids 1 to 580 of SEQ ID NO:
- 104;
- a penton protein of SAdV-48, amino acids 1 to 504 of SEQ ID NO:
- 134;
- a penton protein of SAdV-49, amino acids 1 to 511 of SEQ ID NO:
- 162;
- a penton protein of SAdV-50, amino acids 1 to 511 of SEQ ID NO:
- 188;
- and
- (c) a fiber protein of SAdV-43, amino acids 1 to 599 of SEQ ID NO:
- 22;
- a fiber protein of SAdV-46, amino acids 1 to 353 of SEQ ID NO:
- 89;
- a fiber protein of SAdV-45, amino acids 1 to 601 of SEQ ID NO:
- 59;
- a fiber protein of SAdV-47, amino acids 1 to 322 of SEQ ID NO:
- 119;
- a fiber protein of SAdV-48, amino acids 1 to 570 of SEQ ID NO:
- 147;
- a fiber protein of SAdV-49, amino acids 1 to 521 of SEQ ID NO:
- 174;
- a fiber protein of SAdV-49, amino acids 1 to 418 of SEQ ID NO:
- 175;
- a fiber protein of SAdV-50, amino acids 1 to 521 of SEQ ID NO:
- 200;
- a fiber protein of SAdV-50, amino acids 1 to 418 of SEQ ID NO:
- 201;
- said capsid encapsidating a heterologous molecule carrying a gene operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell.
A recombinant adenovirus having a capsid comprising a hexon containing a fragment of a simian adenovirus (SAdV) hexon protein and a nucleic acid sequence heterologous to the SAdV, wherein the fragment of the SAdV hexon protein is the SAdV hexon protein of SEQ ID NO:
- 11, 48, 109, 139, 167 or 193 with an N-terminal or C-terminal truncation of about 50 amino acids in length or is selected from the group consisting of:
- amino acid residues 125 to 443 of SEQ ID NO:
- 11, 48, 109, 139, 167 or 193;
- amino acid residues 138 to 441 of SEQ ID NO:
- 11, 48, 109, 139, 167 or 193;
- amino acid residues 138 to 163 of SEQ ID NO:
- 11, 48, 109, 139, 167 or 193;
- amino acid residues 170 to 176 of SEQ ID NO:
- 11, 48, 109, 139, 167 or 193;
- and
- amino acid residues 404 to 430 of SEQ ID NO:
- 11, 48, 109, 139, 167 or 193.
A nucleic acid molecule comprising a simian adenovirus (SAdV) nucleic acid selected from the group consisting of:
- SAdV-46 nucleotides 1 to 35608 of SEQ ID NO:
- 69 and its complement;
- SAdV-43 nucleotides 1 to 37189 of SEQ ID NO:
- 1 and its complement;
- SAdV-45 nucleotides 1 to 37152 of SEQ ID NO:
- 38 and its complement;
- SAdV-47 nucleotides 1 to 35563 of SEQ ID NO:
- 99 and its complement;
- SAdV-48 nucleotides 1 to 34201 of SEQ ID NO:
- 129 and its complement;
- SAdV-49 nucleotides 1 to 35499 of SEQ ID NO:
- 157 and its complement;
- SAdV-50 nucleotides 1 to 35512 of SEQ ID NO:
- 183 and its complement;
- and
- a sequence heterologous thereto.
Dependencies are inferred from the claim text.