Methods and compositions for in situ germline genome engineering
AssigneesBoard of Regents of the University of Nebraska, Tokai University Educational System
InventorsChannabasavaiah Gurumurthy, Masato Ohtsuka, Masahiro Sato
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Abstract
Disclosed are methods and compositions for in situ germline genome engineering. The disclosed methods and compositions may be utilized for germline genome engineering in a subject having a reproductive organ containing a fertilized zygote, via: (i) isolating or obtaining the reproductive organ from the subject after a time period following insemination of the subject; (ii) introducing a reagent composition into the reproductive organ, the reagent composition comprising a nuclease system and/or an exogeneous polynucleotide; and (iii) electroporating the reproductive organ.
Claims20
A method for germline genome engineering in a subject having a reproductive organ containing a fertilized zygote comprising:
- (a) isolating or obtaining the reproductive organ from the subject after a time period following insemination of the subject;
- (b) introducing a reagent composition into the reproductive organ, the reagent composition comprising a nuclease system and/or an exogeneous polynucleotide;
- and
- (c) electroporating the reproductive organ.
A reproductive organ containing a fertilized zygote from a subject after a time period following insemination of the subject, the reproductive organ comprising a reagent composition, and the reagent composition comprising a nuclease system and/or an exogenous polynucleotide.
Dependencies are inferred from the claim text.