Haplotypes Associated with Improved Dicamba Tolerance and Glyphosate Tolerance in Transgenic Soybean Plants
AssigneeMonsanto Technology LLC
InventorsMindy L. DeVries Gelder, Paul Feng, Jesse J. Gilsinger, Floyd G. Hancock, Ivan Husic, James Narvel, Curtis W. Scherder, Dean A. Ulbrich
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Abstract
The present invention provides methods and compositions for the identification and selection of loci modulating phenotypic expression of an herbicide tolerance trait in plant breeding. In addition, methods are provided for screening germplasm entries for the performance and expression of this trait.
Claims47
A method of identifying a soybean plant that comprises a genotype associated with dicamba tolerance and reproductive tolerance to glyphosate, comprising:
- detecting in a soybean plant an allele in at least one genetic locus associated with dicamba tolerance and reproductive tolerance to glyphosate, wherein the genetic locus is in a linkage group L genomic region flanked by loci M0205928 (SEQ ID NO:
- 4) and BU765955 (SEQ ID NO:
- 12), and denoting that said plant comprises a genotype associated with dicamba tolerance.
A method for obtaining a soybean plant comprising in its genome at least one dicamba tolerance locus, compromising the steps of:
- (a) genotyping a plurality of soybean plants with respect to at least one genetic locus in a linkage group L genomic region flanked by loci M0205928 (SEQ ID NO:
- 4) and BU765955 (SEQ ID NO:
- 12);
- and
- (b) selecting a soybean plant comprising in its genome at least one genetic locus comprising a genotype associated with dicamba tolerance.
A method for producing a soybean plant comprising in its genome at least one introgressed dicamba tolerance locus comprising the steps of:
- (a) crossing a first soybean plant with a dicamba tolerance locus with a second soybean plant comprising:
- a dicamba sensitivity locus in a first linkage group L genomic region flanked by loci M0205928 (SEQ ID NO:
- 4) and M0129138 (SEQ ID NO:
- 6) and/or at least one polymorphic allele of at least one marker in a second sub-region of said linkage group L region that is flanked by loci BU551363 (SEQ ID NO:
- 9) and BU765955 (SEQ ID NO:
- 12) and/or at least one polymorphic allele of at least one marker in a third sub-region of said linkage group L region that is flanked by loci BU55345 (SEQ ID NO:7) and M0114388 (SEQ ID NO:8) and at least one linked polymorphic locus not present in said first soybean plant to obtain a population segregating for the dicamba tolerance loci and said linked polymorphic locus;
- (b) detecting at least two polymorphic nucleic acids in at least one soybean plant from said population, wherein at least one of said polymorphic nucleic acids is located in said first linkage group L region and/or said second linkage group L region and wherein at least one of said polymorphic amino acids is a linked polymorphic locus not present in said first soybean plant;
- and
- (c) selecting a soybean plant comprising a genotype associated with dicamba tolerance and at least one linked marker found in said second soybean plant comprising a dicamba sensitivity locus but not in said first soybean plant, thereby obtaining a soybean plant comprising in its genome at least one introgressed dicamba tolerance locus.
A transgenic soybean plant comprising introgressed linkage group L regions comprising at least one polymorphic allele of at least one marker in a first sub-region of said linkage group L region that flanked by loci M0205928 (SEQ ID NO:
- 4) and M0129138 (SEQ ID NO:
- 6) and/or at least one polymorphic allele of at least one marker in a second sub-region of said linkage group L region that is flanked by loci BU551363 (SEQ ID NO:
- 9) and BU765955 (SEQ ID NO:
- 12) and/or at least one polymorphic allele of at least one marker in a third sub-region of said linkage group L region that is flanked by loci BU55345 (SEQ ID NO:7) and M0114388 (SEQ ID NO:8), wherein said polymorphic alleles are associated with dicamba tolerance and/or reproductive tolerance to glyphosate, and wherein said plant comprises a transgene that confers resistance to dicamba.
A method of identifying a transgenic soybean plant that comprises a genotype associated with reproductive tolerance to glyphosate, the method comprising:
- (a) scoring at least one transgenic plant in a population of transgenic soybean plants that had been exposed to dicamba for dicamba tolerance, said plants having a transgene that confers resistance to dicamba;
- and,
- (b) selecting a transgenic plant that exhibits dicamba tolerance, thereby identifying a transgenic soybean plant that comprises a genotype associated with reproductive tolerance to glyphosate.
A method of identifying a transgenic soybean plant that comprises a genotype associated with tolerance to dicamba, comprising:
- (a) scoring at least one plant in a population of transgenic soybean plants that had been exposed to glyphosate for reproductive tolerance to glyphosate, wherein said plants comprise a transgene that confers resistance to glyphosate;
- and,
- (b) selecting a transgenic plant that exhibits reproductive tolerance to glyphosate, thereby identifying a transgenic soybean plant that comprises a genotype associated with dicamba tolerance.
A method of obtaining a transgenic soybean plant that comprises a genotype associated with reproductive tolerance to glyphosate, the method comprising:
- (a) exposing a population of transgenic soybean plants to dicamba, wherein said plants have a transgene that confers resistance to dicamba;
- (b) observing dicamba tolerance exhibited by one or more soybean plants following exposure to dicamba;
- and,
- (c) selecting a transgenic plant that exhibits dicamba tolerance, thereby obtaining a transgenic soybean plant that comprises a genotype associated with reproductive tolerance to glyphosate.
Dependencies are inferred from the claim text.