A method for expression of a prokaryotic membrane protein in an eukaryotic organism, products and uses thereof
AssigneeUniversidade Do Minho
InventorsMargarida Paula PEDRA AMORIM CASAL, Isabel João SOARES DA SILVA, Sandra Cristina ALMEIDA PAIVA, David Manuel NOGUEIRA RIBAS, Ana Carolina GOMES ALMEIDA
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Abstract
A method for the production of a functional prokaryotic membrane transporter protein in a eukaryotic host organism comprising the following steps: obtaining a DNA construct by ligating a DNA coding sequence of a prokaryotic membrane transporter protein to the N-terminal and/or C-terminal DNA coding sequences of a eukaryotic membrane protein; introducing the obtained DNA construct in the eukaryotic host organism for the production of the functional prokaryotic membrane transporter protein.
Claims18
A method for the production of a functional prokaryotic membrane transporter protein in a eukaryotic host organism comprising the following steps:
- obtaining a DNA construct by ligating a DNA coding sequence of a prokaryotic membrane transporter protein to a N-terminal coding sequence and/or a C-terminal DNA coding sequence of a eukaryotic membrane protein;
- introducing the obtained DNA construct in the eukaryotic host organism for the production of the functional prokaryotic membrane transporter protein.
The eukaryotic host cell of 16, wherein the eukaryotic host cell increases the tolerance of eukaryotic organisms to intracellular compounds through the export of this molecule.
Dependencies are inferred from the claim text.