Riboswitch-mediated regulatory control of gene expression in thermophilic bacteria
AssigneeAlliance for Sustainable Energy, LLC
InventorsJoan Gabriel MARCANO, Katherine Jenyan CHOU
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Abstract
Methods of riboswitch-mediated gene expression in thermophiles are provided herein.
Claims20
A non-naturally occurring riboswitch operably linked to a promoter wherein the riboswitch is capable of regulating the expression of a gene of interest in a non-naturally occurring thermophilic organism.
A method for regulating the expression of a gene of interest in a non-naturally occurring thermophilic organism comprising operably linking a non-naturally occurring riboswitch to an operator of the gene of interest wherein the expression of the gene of interest is regulated by adding a ligand to the non-naturally occurring thermophilic organism wherein the ligand binds to the non-naturally occurring riboswitch and regulates the expression of the gene of interest.
A method for making ethanol using a thermophilic organism comprising the steps of operably linking a non-naturally occurring riboswitch to an alcohol and aldehyde dehydrogenase and inducing the expression of the alcohol and aldehyde dehydrogenase by adding a ligand to the organism wherein the ligand binds to the non-naturally occurring riboswitch allowing for the expression of the alcohol and aldehyde dehydrogenase.
Dependencies are inferred from the claim text.