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US 2020/0263142 A1Published application
Generation of infectious influenza viruses from virus-like particles
AssigneeWisconsin Alumni Research Foundation (WARF)
InventorsYoshihiro Kawaoka, Gabriele Neumann
Life of this patent
Estimating the term…
Abstract
Compositions and methods to prepare influenza virus-like particles (VLPs) are provided.
Claims13
A set of isolated host cells, comprising:
- a first host cell for producing a first VLP with at least one and up to eight influenza A or B virus segments comprising:
- one or more vectors which include at least one and up to eight transcription cassettes for production of at least one and up to eight viral segments from the genome of influenza A virus selected from:
- a transcription cassette comprising a promoter operably linked to an influenza virus PA DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB1 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB2 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NP DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus M DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NS DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NA DNA;
- or
- a transcription cassette comprising a promoter operably linked to an influenza virus HA DNA or a non-influenza host cell binding protein DNA;
- or
- one or more vectors which include at least one and up to eight transcription cassettes for production of at least one and up to eight viral segments from the genome of influenza B virus selected from:
- a transcription cassette comprising a promoter operably linked to an influenza virus PA DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB1 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB2 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NP DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus M DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NS DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NA and NB DNA;
- or a transcription cassette comprising a promoter operably linked to an influenza virus HA DNA or a non-influenza host cell binding protein DNA;
- and
- one or more vectors which include transcription cassettes for mRNA production including a transcription cassette comprising a promoter operably linked to an influenza virus PA DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB1 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB2 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NP DNA;
- and optionally a transcription cassette comprising a promoter operably linked to an influenza virus HA or a non-influenza host cell binding protein
- and
- a second host cell for producing a second VLP with at least one and up to eight influenza A or B virus viral segments comprising:
- one or more vectors which include at least one and up to eight transcription cassettes for production of at least one and up to eight viral segments from the genome of influenza A selected from:
- a transcription cassette comprising a promoter operably linked to an influenza virus PA DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB1 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB2 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NP DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus M DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NS DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NA DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus HA DNA or a non-influenza host cell binding protein DNA;
- or
- one or more vectors which include at least one and up to eight transcription cassettes for production of at least one and up to eight viral segments from the genome of influenza B selected from:
- a transcription cassette comprising a promoter operably linked to an influenza virus PA DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB1 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB2 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NP DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus M DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NS DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NA and NB DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus HA DNA or a non-influenza host cell binding protein DNA;
- and
- one or more vectors which include transcription cassettes for mRNA production including a transcription cassette comprising a promoter operably linked to an influenza virus PA DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB1 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB2 DNA;
- and a transcription cassette comprising a promoter operably linked to an influenza virus NP DNA;
- and optionally a transcription cassette comprising a promoter operably linked to an influenza virus HA or a non-influenza host cell binding protein,
- wherein the first host cell has at least one transcription cassette for production of at least one viral segment that is not present in the second host cell or wherein the first host cell has at least one transcription cassette for production of at least one viral segment that is modified in the corresponding viral segment in the second host cell so that a functional influenza virus protein is not expressed from the corresponding segment.
52 more elements
An isolated host cell which produces virus-like particles (VLPs) with at least one and up to eight influenza A or B virus segments and has one or more vectors which include at least one and up to eight transcription cassettes for production of at least one and up to eight viral segments from the genome of influenza A virus selected from:
- a transcription cassette comprising a promoter operably linked to an influenza virus PA DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB1 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB2 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NP DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus M DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NS DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NA DNA;
- or
- a transcription cassette comprising a promoter operably linked to an influenza virus HA DNA or a non-influenza host cell binding protein DNA;
- or
- has one or more vectors which include at least one and up to eight transcription cassettes for production of at least one and up to eight viral segments from the genome of influenza B virus selected from:
- a transcription cassette comprising a promoter operably linked to an influenza virus PA DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB1 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB2 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NP DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus M DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NS DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NA and NB DNA;
- o a transcription cassette comprising a promoter operably linked to an influenza virus HA DNA or a non-influenza host cell binding protein DNA;
- and one or more vectors which include transcription cassettes for mRNA production including a transcription cassette comprising a promoter operably linked to an influenza virus PA DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB1 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus PB2 DNA;
- a transcription cassette comprising a promoter operably linked to an influenza virus NP DNA;
- and optionally a transcription cassette comprising a promoter operably linked to an influenza virus HA or a non-influenza host cell binding protein DNA,
- wherein if the host cell has a transcription cassette for only one viral segment, the segment encodes a functional influenza virus protein, wherein if the host cell has a transcription cassette for seven viral segments, the host cell does not include sequences corresponding to NA coding or noncoding sequences for production of a NA viral segment and does not include sequences for mRNA production of NA, and wherein if the host cell has a transcription cassette for eight viral segments, at least one of the segments is modified so that a functional influenza virus protein is not expressed from that segment and wherein the modified segment is not the PB2 segment.
22 more elements
Dependencies are inferred from the claim text.