Engineered host cells and methods of use thereof
AssigneeAgenus Inc.
InventorsMarc VAN DIJK, Volker SEIBERT, Robert Benjamin STEIN
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Abstract
Provided herein are engineered host cells suitable for expression of functional T cell receptors (TCR) and methods of using these cells to identify TCRs that specifically bind to a desired peptide-major histocompatibility complex (MHC) complex.
Claims127
An isolated cell derived from a lymphocyte, the cell comprising a first polynucleotide encoding a recombinant co-receptor, or a first chain thereof, wherein the cell expresses CD3 but does not express endogenous T cell receptor (TCR) on the cell surface.
An isolated cell derived from a lymphocyte, the cell comprising a first polynucleotide encoding a recombinant co-receptor, or a first chain thereof, wherein the lymphocyte is an invariant NKT (iNKT) cell or a diverse NKT (dNKT) cell.
An isolated cell derived from a lymphocyte, the cell comprising a first polynucleotide encoding a recombinant co-receptor, or a first chain thereof, wherein the cell is capable of expressing the recombinant co-receptor, or a first chain thereof, on the cell surface in a regulatable manner.
An isolated cell derived from a mouse lymphocyte, the cell comprising:
- a) a first polynucleotide encoding a chimeric CD8α chain comprising an extracellular region of human CD8α and transmembrane and cytoplasmic regions of mouse CD8α, and
- b) a second polynucleotide encoding a chimeric CD8β chain comprising an extracellular region of human CD8β and transmembrane and cytoplasmic regions of mouse CD8β,
- wherein the cell expresses CD3 but does not express endogenous TCR on the cell surface.
Dependencies are inferred from the claim text.