Methods to Expand a T Regulatory Cell Master Cell Bank
AssigneesThe Trustees of the University of Pennsylvania, Regents of the University of Minnesota
InventorsJames L. Riley, Carl H. June, Bruce R. Blazar, Keli Hippen
Life of this patent
Estimating the term…
Abstract
The present invention provides compositions and methods for expanding natural T regulatory cells (nTregs) without substantially sacrificing suppressive function of the cells. Accordingly, the invention provides uses of the expanded nTregs for cellular therapy.
Claims18
A method for adoptive transfer therapy, the method comprising administering an expanded population of cells comprising natural T regulatory cells (nTregs) to a mammal in need thereof to treat an immune reaction that is adverse to said mammal, wherein said expanded population of cells has been expanded according to a method comprising
- culturing a population of cells comprising nTregs in a culture medium comprising a first agent that provides a primary activation signal to T cells and a second agent that provides a co-stimulatory signal to T cells;
- monitoring proliferation of said nTregs;
- and
- re-stimulating said nTregs when the rate of nTreg proliferation has decreased based upon a desired cell size, thereby inducing further proliferation of said nTregs.
-23. (canceled)
Dependencies are inferred from the claim text.