Compositions and methods for enhanced fluorescence
AssigneesLife Technologies Corporation, Pierce Biotechnology, Inc.
InventorsSurbhi DESAI, Marie Christine NLEND, Kyle GEE, Matthew BAKER, Robert AGGELER, Scott SWEENEY, Aleksey RUKAVISHNIKOV, Shih-Jung HUANG
Life of this patent
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Abstract
This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.
Claims32
-38. (canceled)
A method for increasing fluorescence of a fluorescently labeled biomolecule, the method comprising:
- (a) conjugating a spacer molecule to a biomolecule;
- and
- (b) conjugating a fluorescent label to the biomolecule;
- wherein steps (a) and (b) can be done simultaneously or in any order, and
- wherein the spacer and fluorescent label are not conjugated to each other.
-43. (canceled)
-47. (canceled)
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-61. (canceled)
-65. (canceled)
-71. (canceled)
-122. (canceled)
A method for preparing a fluorescently labeled biomolecule, the method comprising:
- (a) conjugating a reactive group and two or more fluorescent labels to a spacer molecule, thereby forming a fluorescently labeled spacer molecule, and
- (b) conjugating the fluorescently labeled spacer molecule to the biomolecule, thereby forming the fluorescently labeled biomolecule,
- and
- wherein the individual fluorescent labels of the fluorescently labeled biomolecule have a Fluorescent Ratio on a per fluorescent label basis that is equal to or greater than 0.5.
(canceled)
-129. (canceled)
A method for detecting a fluorescently labeled biomolecule, the method comprising:
- (a) exposing the fluorescently labeled biomolecule with light that excites fluorescent labels conjugated to the biomolecule, and
- (b) detecting emitted light produced by the fluorescent labels conjugated to the biomolecule,
- wherein the fluorescently labeled biomolecule is conjugated to four or more fluorescent labels and
- wherein the individual fluorescent labels of the fluorescently labeled biomolecule have a Fluorescent Ratio on a per fluorescent label basis that is equal to or greater than 0.7
(canceled)
Dependencies are inferred from the claim text.