Preservation of biomaterial properties and methods of storing
AssigneeLifeline Scientific, Inc.
InventorKelvin G. M. BROCKBANK
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Abstract
Described herein are enhanced compositions and methods for storing biomaterials. In certain aspects, these biomaterials include natural and engineered eukaryotic tissues. The methods described herein include storing these biomaterials in such a manner that reduces or prevents the loss of biomaterial properties (e.g., extracellular matrix integrity, cell viability, or a combination thereof) occurring either during storage or after removal of the biomaterial from storage. In certain aspects, these biomaterials will be stored in animal product-free solutions containing an agent that prevents or reduces the loss of extracellular matrix integrity.
Claims18
A method for storing a biomaterial comprising:
- preparing the composition by placing a biomaterial in a solution that includes at least one agent that reduces or prevents a loss of biomaterial properties, wherein
- the solution is an animal product-free solution,
- the biomaterial comprises chondrocytes in an extracellular matrix or cartilage, and the at least one agent comprises doxycycline having a concentration ranging from 10 μM to 30 μM, and
- wherein said biomaterial properties comprise cell viability and extracellular matrix integrity, said extracellular matrix integrity including extracellular matrix permeability.
Dependencies are inferred from the claim text.