Host cells and methods for reducing isoprenoid precursors and isoprenoids by geranylgeranyl reductase
AssigneeThe Regents of the University of California
InventorsCorey Meadows, Brett Garabedian, Florence Mingardon, Angelique Chanal, Taek Soon Lee
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Abstract
The present invention provides for a genetically modified host cell capable of reducing one or more isoprenoid, or precursor thereof, said genetically modified host cell comprising one or more geranylgeranyl reductases (GGRs), or polypeptides comprising an amino acid sequence having at least 70% identity to an amino acid sequence of a geranylgeranyl reductase (GGR), wherein the polypeptide comprises the enzymatic activity for catalyzing one or more GGR catalyzed reactions, and/or reducing one or more C15 or C20 prenyl alcohols or prenyl pyrophosphates.
Claims4
A genetically modified host cell capable of reducing one or more isoprenoid, or precursor thereof, said genetically modified host cell comprising one or more geranylgeranyl reductases (GGRs), or polypeptides comprising an amino acid sequence having at least 70% identity to an amino acid sequence of a geranylgeranyl reductase (GGR) of Table 1 of Example 1, or Table 1 of Example 2, wherein the polypeptide comprises the enzymatic activity for catalyzing one or more of the GGR catalyzed reactions depicted in FIG. 1 of Example 1, and/or Scheme 1, and/or reducing one or more C15 or C20 prenyl alcohols or prenyl pyrophosphates.
An isolated or purified geranylgeranyl reductase (GGR) of Table 1 of Example 1, or Table 1 of Example 2.
A geranylgeranyl reductase (GGR), or a polypeptide comprising an amino acid sequence having at least 70% identity to an amino acid sequence of a geranylgeranyl reductase (GGR) of Table 1 of Example 1, or Table 1 of Example 2, comprising one or more mutations in the amino acid residue which corresponds to L377, D82, Q84, D207, E209, P212, N359, K367, G298, G299, G300, A304, S307, or G308 of Sulfolobus acidocaldarius GRR (SaGRR), or any other amino acid residue described herein.
A method for reducing one or more isoprenoid, or precursor thereof, comprising:
- (a) providing a genetically modified host cell of the present invention, or a culture comprising the genetically modified host cell, (b) culturing the genetically modified host cell to produce one or more isoprenoid, or precursor thereof, and expressing the geranylgeranyl reductase (GGR), or polypeptide, and (c) reducing the one or more isoprenoid, or precursor thereof, by the geranylgeranyl reductase (GGR), or polypeptide.
Dependencies are inferred from the claim text.