A method for the production of a dairy food product and a method for gene transfer by conjugation
AssigneeDsm IP Assets B.V.
InventorsMariya Alexeevna TARAZANOVA, Thom HUPPERTZ, Herwig BACHMANN, Jan KOK, Marke BEERTHUYZEN
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Abstract
The present invention relates to the field of microbiology, specifically the field of production of a dairy food product using fermentation and the field of gene transfer by conjugation.
Claims25
A dairy bacterial strain with clumping and/or chaining properties, optionally when cultured under liquid conditions, optionally without a substantial amount of a clumping agent, wherein the clumping property results in clumps of at least 20 bacteria per average clump and wherein the chaining property results in chains of at least 8 bacteria per average chain.
A bacterial strain comprising an increased amount of expression product of a pilin gene cluster, wherein the amount of expression product is increased in view of Lactococcus lactis subsp cremoris NCDO712.
A method for the production of a desired bacterial strain comprising a genetic trait of interest, comprising providing a composition comprising:
- a precursor of the desired bacterial strain, wherein the precursor lacks the genetic trait of interest, and
- a donor bacterial strain comprising the genetic trait of interest,
- incubating the composition under conditions conducive to conjugation, and optionally isolating the desired bacterial strain comprising the genetic trait of interest, wherein at least one bacterial strain in the composition comprises a pilin gene cluster.
Dependencies are inferred from the claim text.