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AssigneeHyasynth Biologicals Inc.
InventorsShoham Mookerjee, Alexander James Campbell, Zachary Douglas Wiltshire, Kevin John Chen
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A method and cell line for producing polyketides in yeast. The method applies, and the cell line includes, a yeast cell transformed with a polyketide synthase coding sequence. The polyketide synthase enzyme catalyzes synthesis of olivetol or methyl-olivetol, and may include Dictyostelium discoideum polyketide synthase (“DiPKS”). Wild type DiPKS produces methyl-olivetol only. DiPKS may be modified to produce olivetol only or a mixture of both olivetol and methyl-olivetol. The yeast cell may be modified to include a phosphopantethienyl transferase for increased activity of DiPKS. The yeast cell may be modified to mitigate mitochondrial acetaldehyde catabolism for increasing malonyl-CoA available for synthesizing olivetol or methyl-olivetol.
A method of producing a polyketide, the method comprising:
A polynucleotide comprising a coding sequence for a DiPKS polyketide synthase enzyme from D. discoideum with a primary structure with between 80% and 100% amino acid residue sequence identity with a protein coded for by a reading frame defined by bases 535 to 9978 of SEQ ID NO:13, with a charged amino acid residue at amino acid residue position 1516 in place of a glycine residue at position 1516 for mitigating methylation of at least one species of polyketide synthesized by the polyketide synthase.
A DiPKS polyketide synthase enzyme from D. discoideum with a primary structure with between 80% and 100% amino acid residue sequence identity with a protein coded for by a reading frame defined by bases 535 to 9978 of SEQ ID NO:13, with a charged amino acid residue at amino acid residue position 1516 in place of a glycine residue at position 1516 for mitigating methylation of at least one species of polyketide synthesized by the polyketide synthase.
Dependencies are inferred from the claim text.