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US 2020/0239833 A1Published application
Use of magnetic cells to manipulate non-magnetic cells
AssigneeGreiner Bio-One North America, Inc.
InventorGlauco SOUZA
Life of this patent
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Abstract
Methods of using magnetic display cells to replace secondary antibodies and magnetic beads in any cell manipulation methods. Cells displaying ligands for target cells are magnetized, and then used to bind the target cells. The complex can then be collected in a magnetic field, and thereby manipulated according to the application needs.
Claims18
1) A method of isolating target cells from a mixture of cells, comprising:
- a) combining a mixture of cells comprising target cells and non-target cells with magnetized display cells that display one or more ligands on their surfaces, said ligands specific for a binding partner on a surface of said target cells;
- b) allowing said ligand to bind to said binding partner to produce magnetized display cells bound to said target cells;
- c) applying a magnetic field to collect said magnetized display cells bound to said target cells;
- and
- d) washing away non-target cells, thereby isolating said target cells.
2 more elements
11) A method of isolating target cells from a mixture of cells, comprising:
- a) combining cells displaying one or more antigen binding site(s) on their surfaces with a composition to form magnetized display cells, said composition comprising:
- i) a negatively charged nanoparticle;
- ii) a positively charged nanoparticle;
- and
- iii) a support molecule;
- iv) wherein one of said negatively charged nanoparticle or positively charged nanoparticle is a magnetically responsive element or compound, and wherein said support molecule holds said negatively charged nanoparticle and said positively charged nanoparticle in an intimate admixture forming a fibrous mat-like structure;
- b) combining a mixture of cells comprising target cells and non-target cells with said magnetized display cells;
- c) allowing said antigen binding site(s) to bind to one or more target cell(s) displaying an antigen that is recognized by said antigen binding site(s);
- d) applying a magnetic field to collect magnetized display cells bound to said target cells;
- and
- e) washing away non-target cells, thereby isolating magnetized display cells bound to said target cells.
8 more elements
15) A method of manipulating target cells, comprising:
- a) combining target cells with magnetized display cells that display one or more ligands on their surfaces, said ligands for specifically binding a target molecule on a surface of said target cells;
- b) allowing said ligands to bind to said target molecule to produce magnetized display cells bound to said target cells;
- c) applying a magnetic field to collect said magnetized display cells bound to said target cells, thereby allowing manipulation of said target cells.
16) A magnetic cell complex comprising:
- a) at least one magnetized display cell displaying at least one ligand on a surface of the at least one magnetized display cell;
- and
- b) at least one target cell having at least one binding partner on a surface of the at least one target cell,
- wherein said at least one ligand on the surfaces of the at least one magnetized display cell binds said binding partner on the surface of the at least one target cell so as to form the magnetic cell complex.
1 more element
Dependencies are inferred from the claim text.