Method for growing somatic embryos of conifers into trees
AssigneeUniversity of Copenhagen
InventorJens Iver FIND
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Abstract
The present invention relates to a method for producing trees or wood from fully mature cotyledonary somatic embryos (SE) of conifer species within the genus Abies and to the use of plants thus obtained. The invention concerns a method for treating and growing somatic embryos under conditions that induce and stimulate root growth, that induce and stimulate shoot formation and increases the survival rate of the embryos, plantlets and emblings. The emblings can subsequently be grown into trees. Trees derived from somatic embryogenesis and grown according to the method of the invention are of a very homogeneous appearance and quality, which is preferred in the production of trees such as Christmas trees thereby reducing the wastage rate.
Claims16
1-21. (canceled)
A method for development of plants from fully mature somatic embryos of the genus Abies comprising:
- a) subjecting fully mature somatic embryos to 2° C.-7° C. for 8-16 weeks;
- b) selecting the small plantlets obtained from step (a) that have developed a root and growing said small plantlets in a substrate that does not comprise a significant concentration of a plant accessible carbohydrate source;
- at LED light intensities of 70-300 μmol/m2s for at least 3 weeks;
- and
- c) selecting the emblings obtained from step b) and growing these into plants.
The method according to claim 6 wherein said LED light intensities in step b) are 175-225 μmol/m2s.
Dependencies are inferred from the claim text.