Methods and compositions for fluorescent and colorimetric protein quantitation

A composition comprising:

• acetonitrile;
• and
• a reagent having general formula (I):
• where,
• each of R1, R2, R3, R4, R5 and R6 is independently alkyl including but not limited to a C1-C6 straight or branched alkyl or a C6-C20 aryl, alkylaryl, or arylalkyl such as methyl (—CH3), ethyl (—CH2CH3), propyl (—CH2CH2CH3), butyl (—CH2CH2CH2CH3) or phenyl (—C6H5);
• each of R3, R4, R5 and R6 is also additionally independently selected from the group consisting of hydrogen (H), sulfonate (—SO3−) salt of sodium (Na+), sulfonate (—SO3) salt of potassium (K+), sulfonate (—SO3−) salt of lithium (Li+), phosphonate (—PO3−) salt of sodium (Na+), phosphonate (—PO3−) salt of potassium (K+), phosphonate (—PO3−) salt of lithium (Li+), carboxylate (—CO2−) salt of sodium (Na+), carboxylate (—CO2) salt of potassium (K+), and carboxylate (—CO2−) salt of lithium (Li+);
• wherein when R5 and R6 is a phenyl (—C6H5), the phenyl (—C6H5) can additionally independently have attached to it a molecule selected from the group consisting of sulfonate (—SO3−) salt of sodium (Na+), sulfonate (—SO3−) salt of potassium (K+), sulfonate (—SO3−) salt of lithium (Li+);
• phosphonate (—PO3−) salt of sodium (Na+), phosphonate (—PO3−) salt of potassium (K+), phosphonate (—PO3−) salt of lithium (Li+), carboxylate (—CO2−) salt of sodium (Na+), carboxylate (—CO2−) salt of potassium (K+) and carboxylate (—CO2−) salt of lithium (Li+);
• and
• wherein the reagent is present in a hydrated, ((I) H2O), or a non-hydrated form.

A kit comprising:

• 1) a composition comprising acetonitrile and a reagent having the general formula (I):
• where,
• each of R1, R2, R3, R4, R5 and R6 is independently alkyl including but not limited to a C1-C6 straight or branched alkyl or a C6-C20 aryl, alkylaryl, or arylalkyl such as methyl (—CH3), ethyl (—CH2CH3), propyl (—CH2CH2CH3), butyl (—CH2CH2CH2CH3) or phenyl (—C6H5);
• each of R3, R4, R5 and R6 is also additionally independently selected from the group consisting of hydrogen (H), sulfonate (—SO3−) salt of sodium (Na+), sulfonate (—SO3) salt of potassium (K+), sulfonate (—SO3−) salt of lithium (Li+), phosphonate (—PO3−) salt of sodium (Na+), phosphonate (—PO3−) salt of potassium (K+), phosphonate (—PO3−) salt of lithium (Li+), carboxylate (—CO2−) salt of sodium (Na+), carboxylate (—CO2−) salt of potassium (K+), and carboxylate (—CO2−) salt of lithium (Li+);
• wherein when R5 and R6 is a phenyl (—C6H5), the phenyl (—C6H5) can additionally independently have attached to it a molecule selected from the group consisting of sulfonate (—SO3−) salt of sodium (Na+), sulfonate (—SO3−) salt of potassium (K+), sulfonate (—SO3−) salt of lithium (Li+);
• phosphonate (—PO3−) salt of sodium (Na+), phosphonate (—PO3−) salt of potassium (K+), phosphonate (—PO3−) salt of lithium (Li+), carboxylate (—CO2−) salt of sodium (Na+), carboxylate (—CO2−) salt of potassium (K+) and carboxylate (—CO2−) salt of lithium (Li+);
• and
• wherein the reagent is present in a hydrated, ((I) H2O), or a non-hydrated form;
• and
• 2) copper;
• each ingredient contained in one or more separate containers.