Bioproduction of phenethyl alcohol, aldehyde, acid, amine, and related compounds

An isolated strain of genetically engineered cells capable of increased bioproduction of substituted or unsubstituted phenylacetaldehyde, 2-phenylethanol, phenylacetic acid or phenylethylamine in a one-pot reaction system compared to wild type cells, wherein the cells overexpress a combination of enzymes selected from the group (i) to (iv) comprising:

• i) styrene monooxygenase and styrene oxide isomerase for generating substituted or unsubstituted phenylacetaldehyde from styrene or substituted styrene;
• ii) styrene monooxygenase, styrene oxide isomerase and an aldehyde dehydrogenase for generating substituted or unsubstituted phenylacetic acid from styrene or substituted styrene;
• iii) styrene monooxygenase, styrene oxide isomerase, an aldehyde reductase and/or an alcohol dehydrogenase for generating substituted or unsubstituted 2-phenylethanol from styrene or substituted styrene;
• and
• iv) styrene monooxygenase, styrene oxide isomerase and a transaminase for generating substituted or unsubstituted phenylethylamine from styrene or substituted styrene.

An isolated nucleic acid molecule encoding at least one catalytic enzyme selected from a group (i)-(viii) comprising:

• (i) a nucleic acid encoding styrene monooxygenase and styrene oxide isomerase for generating substituted or unsubstituted phenylacetaldehyde from styrene or substituted styrene;
• (ii) a nucleic acid encoding styrene monooxygenase, styrene oxide isomerase and an aldehyde dehydrogenase for generating substituted or unsubstituted phenylacetic acid from styrene or substituted styrene;
• (iii) a nucleic acid encoding styrene monooxygenase, styrene oxide isomerase, an aldehyde reductase and/or an alcohol dehydrogenase for generating substituted or unsubstituted 2-phenylethanol from styrene or substituted styrene;
• (iv) a nucleic acid encoding styrene monooxygenase, styrene oxide isomerase and a transaminase for generating substituted or unsubstituted phenylethylamine from styrene or substituted styrene;
• (v) a nucleic acid encoding at least one heterologous catalytic enzyme selected from ammonia lyase and decarboxylase for generating styrene or substituted styrene from L-phenylalanine or substituted L-phenylalanine;
• (vi) a nucleic acid encoding at least one heterologous catalytic enzyme selected from ammonia lyase and decarboxylase, styrene monooxygenase, styrene oxide isomerase, an aldehyde reductase and/or an alcohol dehydrogenase or mutants thereof to transform a L-phenylalanine or substituted L-phenylalanine to a substituted or unsubstituted 2-phenylethanol;
• (vii) a nucleic acid encoding at least one heterologous catalytic enzyme selected from a group comprising DAHP synthase (AroG) or AroG*, shikimate kinase (AroK), shikimate dehydrogenase (YdiB), chorismate mutase/prephenate dehydratase (PheA) or PheA* and tyrosine aminotransferase (TyrB), or mutants thereof for generating L-phenylalanine from glucose;
• and
• (viii) a nucleic acid encoding at least one heterologous catalytic enzyme selected from a group comprising DAHP synthase (AroG) or AroG*, shikimate kinase (AroK), shikimate dehydrogenase (YdiB), chorismate mutase/prephenate dehydratase (PheA) or PheA* and tyrosine aminotransferase (TyrB), ammonia lyase and decarboxylase, styrene monooxygenase, styrene oxide isomerase, an aldehyde reductase and/or an alcohol dehydrogenase or mutants thereof to transform glucose to a substituted or unsubstituted 2-phenylethanol.