US 2020/0225235 A1 · SENSITIVE DETECTION OF G-PROTEIN COUPLED RECEPTOR-ASSOCIATED SORTING PROTEIN 1 (GASP-1), GASP-1 MICROVESICLES, AND GASP-1 EXOSOMES

Abstract

A highly sensitive method of detecting GASP-1 or a fragment thereof in a sample is provided. The method comprises (a) exposing a surface to a sample comprising GASP-1 or a fragment thereof; (b) immobilizing an anti-GASP-1 detection antibody to the surface; (c) measuring the amount of the anti-GASP-1 detection antibody immobilized to the surface; and (d) determining the presence of the GASP-1 or a fragment thereof in the sample based on the amount of the anti-GASP-1 detection antibody immobilized to the surface. A coating agent may be immobilized to the surface in step (a), and the anti-GASP-1 detection antibody may be immobilized to the surface via the coating agent, directly or indirectly. The coating agent may be selected from the group consisting of a first GASP-1 fragment, a conjugate of a protein (e.g., bovine serum albumin (BSA)) and a GASP-1 peptide, a capture antibody against a microvesicle or exosomal surface biomarker, a capture antibody against a second GASP-1 fragment, and a combination thereof. Also provided are kits for detecting GASP-1 or its fragment.

Claims20

Dependent claims

A method of detecting G protein coupled receptor-associated sorting 1 (GASP-1) or a fragment thereof in a sample, comprising:

(a) exposing a surface to a sample comprising GASP-1 or a fragment thereof, wherein a coating agent is immobilized to the surface and is selected from the group consisting of a first GASP-1 fragment, a conjugate of a protein and the first GASP-1 fragment, a capture antibody against a microvesicle or exosomal surface biomarker, a capture antibody against a second GASP-1 fragment, and a combination thereof, wherein the first and second GASP-1 fragments are different;
(b) adding an anti-GASP-1 detection antibody against the first GASP-1 fragment, wherein the anti-GASP-1 detection antibody has a concentration of 0.01-1 ng/ml, whereby the anti-GASP-1 detection antibody is immobilized to the surface via the coating agent;
(c) measuring the amount of the anti-GASP-1 detection antibody immobilized to the surface;
and
(d) determining the presence of the GASP-1 or a fragment thereof in the sample based on the amount of the anti-GASP-1 detection antibody immobilized to the surface.

2 more elements

Dependencies are inferred from the claim text.

Sensitive detection of g-protein coupled receptor-associated sorting protein 1 (gasp-1), gasp-1 microvesicles, and gasp-1 exosomes

Life of this patent

Sensitive detection of g-protein coupled receptor-associated sorting protein 1 (gasp-1), gasp-1 microvesicles, and gasp-1 exosomes

Life of this patent

Abstract

Claims20