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US 2020/0224230 A1Published application
Methionine-producing yeast
AssigneeAdisseo France S.A.S.
InventorsDominique LOUIS, Karine JAILLARDON, Dominique THOMAS
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Abstract
A method of the bio-production of methionine and/or of its derivatives thereof from a reduced source of sulfur, such as MeSH or MeSNa including genetically modified yeasts, having an increased ability to produce methionine and/or its derivatives thereof, as compared to the parent yeasts.
Claims25
A methionine-producing and/or methionine derivatives-producing recombinant yeast, in the genome of which:
- (A) at least one nucleic acid encoding an aspartate semi-aldehyde dehydrogenase and/or at least one nucleic acid encoding an aspartate semi-aldehyde dehydrogenase that can use as coenzyme both NAD and NADP is overexpressed and/or is under the control of an inducible or repressible promoter;
- (B) at least one nucleic acid encoding an aspartokinase is under the control of an inducible or repressible promoter;
- and
- (C) (i) a) at least one nucleic acid encoding an homoserine-O-acetyltransferase MET2 is overexpressed and/or is under the control of an inducible or repressible promoter;
- and/or at least one nucleic acid encoding an homoserine-O-acetyltransferase METX is overexpressed and/or is under the control of an inducible or repressible promoter, and
- b) at least one nucleic acid encoding a methionine synthase is overexpressed and/or is under the control of an inducible or repressible promoter;
- and/or
- ii) a) at least one nucleic acid encoding an homoserine kinase is overexpressed and/or is under the control of an inducible or repressible promoter, and
- b) at least one nucleic acid encoding a cystathionine gamma-synthase 1 that has an improved O-phospho-L-homoserine (OHPS) dependent methionine synthase activity is overexpressed and/or is under the control of an inducible or repressible promoter.
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Dependencies are inferred from the claim text.