US 2020/0224179 A1 · Carotenoid oxygenase and its application

Abstract

A protease with a function of degrading carotenoids of scallop is provided, which belongs to the technical field of genetic engineering. In particular, the present invention relates to the sequence and application of a carotenoid oxidase gene of scallop. The present invention analyzes the gene sequence, and verifies that the gene has function of degrading carotenoids of scallop scallop by RNA interference technology, and can be used to increase the carotenoid content of the scallop muscle of scallop scallop. The gene verified that the gene expression products can degrade carotenoids accumulated in bacteria. In addition, the RNA interference method provides a powerful tool for the study of scallop functional genes; the prokaryotic expression and protein purification methods provided can efficiently prepare oxidative lyases of carotenoid in scallop, so as to provide necessary experimental tools for in-depth research of the mechanism of shell carotene metabolism.

Claims10

Dependent claims

A protease, comprising:

(1) a protease with an amino acid sequence of SEQ ID NO:
1;
(2) a homologous protease which is substituted, deleted, or added with one or more amino acids in the amino acid sequence of (1) and has protease activity of the protease in (1);
and
(3) an allele enzyme of the protease with the amino acid sequence of the SEQ ID NO:
1, which is screened from Patinopecten yessoensis.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/828,980Filed 25 Mar 2020

Priority

CN 201910225151X25 Mar 2019

Published

16 Jul 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12N 9/0069
Oxidoreductases: acting on single donors with incorporation of molecular oxygen, i.e. oxygenases
C12N 15/52
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: genes encoding for enzymes or proenzymes
C12P 23/00
Preparation of compounds containing a cyclohexene ring having an unsaturated side chain
C12Y 113/11051
With incorporation of two atoms of oxygen: 9-Cis-epoxycarotenoid dioxygenase

Carotenoid oxygenase and its application

Life of this patent

Carotenoid oxygenase and its application

Life of this patent

Abstract

Claims10