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US 2020/0222550 A1Published application
Methods for production of capsular polysaccharide protein conjugates from streptococcus pneumoniae serotype 19f
AssigneeMerck Sharp & Dohme Corp.
InventorsMichael Albert Winters, John E. MacNair
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Abstract
The present invention provides a method of producing a polysaccharide-protein conjugate with capsular polysaccharide from Streptococcus pneumoniae serotype 19F conjugated to a carrier protein. The method includes a prolonged incubation step prior to filtration to remove free polysaccharide.
Claims18
A method for the production of a polysaccharide-protein conjugate comprising Streptococcus pneumoniae serotype 19F capsular polysaccharide covalently linked to a carrier protein from a mixture comprising polysaccharide-protein conjugate and free polysaccharide, the method comprising the steps of:
- a) incubating said mixture for a minimum of 6 hours at a temperature ranging from 2-30° C. in a buffer having a pH in the range of 5.0 to 9.0;
- and
- b) performing size separation under conditions that allow removal of free polysaccharide.
Dependencies are inferred from the claim text.