Processive enzyme molecular electronic sensors for dna data storage
AssigneeRoswell Biotechnologies, Inc.
InventorsBarry Merriman, Tim Geiser, Paul Mola, Gina Costa
Life of this patent
Estimating the term…
Abstract
A processive enzyme molecular sensor for use in a DNA data storage system is disclosed that can extract digital information suitably encoded into a synthetic DNA molecule. In various aspects, such sensors are provided in a high-density chip-based format that can provide the high throughput, low-cost and fast data extraction capability required for large scale DNA data storage systems. The sensor for reading the digital data stored in DNA molecules processes individual encoded DNA molecules directly, eliminating the need for complicated sample preparation such as making copies of DNA or clonal populations of such molecules.
Claims40
A sensor comprising:
- a first electrode;
- a second electrode spaced apart from the first electrode by an electrode gap;
- a processive enzyme conjugated to the first and second electrodes, the processive enzyme comprising a native or genetically engineered polymerase, reverse transcriptase, helicase, exonuclease, or molecular motor for packaging of viral DNA;
- and
- a trans-impedance amplifier electrically connected to at least one of the first electrode and second electrode, the trans-impedance amplifier providing an output comprising a measurable electrical parameter;
- wherein the measurable electrical parameter comprises distinguishable signals corresponding to enzymatic activity of the processive enzyme.
A method of reading encoded information, the method comprising:
- translocating a synthetic DNA molecule through a processive enzyme, the processive enzyme electrically connected in a molecular sensor circuit;
- and
- generating signals in a measureable electrical parameter of the circuit,
- wherein the signals correspond to the encoded information.
A DNA information system comprising:
- a synthetic DNA molecule comprising a DNA template strand and distinguishable signaling features bonded thereon, the distinguishable signaling features encoding information in the synthetic DNA molecule;
- a buffer solution in contact with the synthetic DNA molecule, the first and second electrodes, and the processive enzyme;
- and
- a sensor capable of reading the information encoded in the synthetic DNA molecule, the sensor comprising:
- a first electrode;
- a second electrode spaced apart from the first electrode by an electrode gap;
- a processive enzyme conjugated to both the first and second electrodes;
- and a trans-impedance amplifier electrically connected to at least one of the first and second electrodes, the trans-impedance amplifier providing an output comprising a measurable electrical parameter,
- wherein the measurable electrical parameter comprises distinguishable signals corresponding to the encoded information.
Dependencies are inferred from the claim text.