Method for producing platelets
AssigneesKyoto University, Megakaryon Corporation
InventorsKOJI ETO, SOU NAKAMURA, YUKITAKA ITO
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Abstract
Healthy functional platelets are mass produced. A method for producing platelets, comprising: (1) a culture step of culturing megakaryocytes in a platelet producing medium in the presence of mechanical stress and platelet production promoting factors including MIF, NRDc, IGFBP2, TSP-1, PAI-1, and CCL5, and (2) a harvest step of harvesting the platelets obtained by the culture step; wherein the culture step comprises: (a) a step of promoting a release of the platelet production promoting factors from megakaryocytes by mechanical stress; and/or (b) a step of externally adding platelet production promoting factors including MIF, NRDc, and IGFBP2.
Claims15
-11. (canceled)
A method for producing platelets, comprising the steps of:
- (1) culturing megakaryocytes in a platelet producing medium, and
- (2) harvesting platelets obtained by the culturing;
- wherein the step of culturing comprises at least one of:
- (a) promoting a release of one or more platelet production promoting factors selected from MIF, NRDc, IGFBP2, TSP-1, PAI-1, and CCL5, from megakaryocytes by applying mechanical stress;
- and
- (b) externally adding one or more platelet production promoting factors selected from MIF, NRDc, and IGFBP2.
A platelet production promoting agent comprising platelet production promoting factors including at least MIF, NRDc, and IGFBP2.
Dependencies are inferred from the claim text.