Effective vaccination against porcine reproductive and respiratory syndrome (prrs) virus prior to weaning
AssigneeZoetis Services LLC
InventorsJay Gregory Calvert, Robert G. Ankenbauer, Jacqueline Gayle Marx, Douglas S. Pearce, Marcia L. Keith
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Abstract
The invention provides isolated polynucleotide molecules that comprise a DNA sequence encoding an infectious RNA sequence encoding a genetically-modified North American PRRS virus, methods to make it and related polypeptides, polynucleotides, and various components. Vaccines comprising the genetically modified virus and polynucleotides and a diagnostic kit to distinguish between naturally infected and vaccinated animals are also provided.
Claims6
A vaccine for protecting a porcine animal against infection by a PRRS virus, which vaccine comprises (a) a North American PRRS virus encoded by the polynucleotide molecule of SEQ ID NO:
- 6 or any polynucleotide which hybridizes thereto under highly stringent conditions, hybridization to filter bound DNA in 0.5 M NaHPO4. 7% SDS, 1 mM EDTA at 65 degrees C., and washing in 0.1×SSC/0.1% SDS at 68 degrees C., (b) said encoding polynucleotide molecule, (c) said polynucleotide molecule in the form of a plasmid, or (d) a viral vector comprising said polynucleotide molecule, wherein the PRRS virus is able to elicit an effective immunoprotective response against infection by PRRS virus, in an amount effective to produce immunoprotection against infection, and a carrier suitable for veterinary use, and wherein said vaccine provides early and safe vaccination as early as when the piglet is 1 day of age, and wherein said vaccine provides a duration of immunity for up to 6 months.
A method for vaccinating a porcine animal against infection by a PRRS virus, comprising administering said vaccine between about 12 hours after birth and 2 weeks of age, wherein said vaccine comprises
- (a) an isolated polynucleotide molecule comprising a DNA sequence encoding an infectious RNA molecule encoding a North American PRRS virus,
- (b) an infectious RNA molecule encoding a North American PRRS virus;
- (c) said polynucleotide molecule (a) in the form of a plasmid, or
- (d) a viral vector comprising an infectious sequence.
Dependencies are inferred from the claim text.