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US 2020/0208178 A1Published application
Production of beta-phellandrene using genetically engineered photosynthetic microorganisms
AssigneeThe Regents of the University of California
InventorsAnastasios MELIS, Fiona K. Davies, Hsu-Ching Chen Wintz, Andreas Zurbriggen
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Abstract
The present invention provides methods and compositions for producing β-phellandrene hydrocarbons from a photosynthetic microorganism such as cyanobacteria.
Claims15
A method of obtaining β-phellandrene from photosynthetic microorganisms, the method comprising:
- culturing a strain of a photosynthetic microorganism that has been genetically modified to express a heterologous β-phellandrene synthase under conditions in which the β-phellandrene synthase is expressed;
- and
- collecting from the surface of the culture medium β-phellandrene hydrocarbons that have spontaneously diffused into the medium from the photosynthetic microorganisms across the cell wall, wherein the culture is in a continuous growth phase and the β-phellandrene hydrocarbons are continuously generated.
A cell culture comprising a photosynthetic microorganism, wherein the photosynthetic microorganism is genetically modified to express a heterologous β-phellandrene synthase;
- and cell culture media comprises β-phellandrene produced by the photosynthetic microorganism that has diffused into the cell culture media from the photosynthetic microorganism and floats on the surface of the culture medium, wherein the cell culture is in a continuous growth phase and the β-phellandrene hydrocarbons are continuously generated.
Dependencies are inferred from the claim text.