Hydrodynamic focusing apparatus and methods
AssigneeCytonome/St, LLC
InventorsErin Koksal, Johnathan Charles Sharpe, Kristopher Scott Buchanan, Blair D. Morad
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Abstract
A microfluidic chip having a micro channel for processing a sample is provided. The micro channel may focus the sample by using focusing fluid and a core stream forming geometry. The core stream forming geometry may include a lateral fluid focusing component and one or more vertical fluid focusing components. A microfluidic chip may include a plurality micro channels operating in parallel on a microfluidic chip.
Claims17
A microfluidic assembly for use with a particle processing instrument, the microfluidic assembly comprising:
- a substrate;
- and
- a flow channel formed in the substrate, the flow channel having:
- an inlet configured to receive a sample stream;
- a fluid focusing region configured to focus the sample stream, the fluid focusing region having a lateral fluid focusing feature, a first vertical fluid focusing feature, and a second vertical fluid focusing feature, wherein a bottom surface of the flow channel lies in a first plane upstream of the first and second vertical fluid focusing features and the bottom surface of the flow channel shifts vertically upward to lie in a second plane downstream of the first and second vertical focusing features;
- and
- an inspection region at least partially downstream of the fluid focusing region.
A microfluidic assembly for use with a particle processing instrument, the microfluidic assembly comprising:
- a substrate;
- and
- a flow channel formed in the substrate, the flow channel having:
- an inlet configured to receive a sample stream;
- and
- a fluid focusing region configured to focus the sample stream, the fluid focusing region having a lateral fluid focusing feature, a first vertical fluid focusing feature, and a second vertical fluid focusing feature, wherein a top surface of the flow channel lies in a first plane upstream of the first and second vertical fluid focusing features and the top surface of the flow channel shifts vertically upward to lie in a second plane downstream of the first and second vertical focusing features.
Dependencies are inferred from the claim text.