Engineered variants of hiv-1 env for presentation of quartenary epitopes
AssigneeThe Board of Trustees of the University of Illinois
InventorsErik PROCKO, Jeremiah Dallas HEREDIA
Life of this patent
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Abstract
Provided herein are HIV-1 Env proteins or fragments thereof comprising one or more amino acid mutations; and nucleic acid molecule encoding the same. Further provided is a method of screening a compound for binding to one or more mutant HIV-1 Env proteins; and methods for eliciting an immune response against an HIV-1 infected cell, comprising administering to a subject an amount of a mutant HIV-1 Env protein, a fragment thereof a mutant HIV-1 Env trimeric complex, or portion thereof, effective to elicit an immune response in the subject. Further provided is a pharmaceutical composition, such as a vaccine, comprising the mutant HIV-1 Env protein or fragment thereof.
Claims26
An HIV-1 Env protein or fragment thereof comprising one or more of the amino acid mutations listed below, wherein the amino acids are numbered by HXB2 numbering:
- TABLE 1
- T49D
- Q114A
- K117V
- K117Y
- P124D
- T163D
- R166E
- R166F
- R166L
- I181L
- V181L
- V200E
- V200T
- A200E
- V208M
- F223Y
- V254T
- V255M
- T283P
- R315Q
- R315A
- F382W
- K315A
- R432T
- Y484W
- G514P
- G516Q
- R557Q
- L581D
- I595M
- L663N
An HIV-1 Env protein or fragment thereof comprising one or more of the sets of amino acid mutations listed below, wherein the amino acids are numbered by HXB2 numbering:
- TABLE 2
- V200E + F223Y
- R432T + R557Q
- R315A + L663N
- Q114A + V200T
- K117V + T163D
- K117V + R315A
- Q114A + L663N
- V200T + I595M
- T49D + R315A + I595M
- R166L + F223Y + L663N
- K117V + R166L + R315A
- K117V + R166L + F223Y
- T163D + V200T + L581D
- R166L + R315A + G514P
- R315A + L663N + T49D
- R315A + L663N + R166L
- R315A + L663N + F223Y
- R315A + L663N + R432T
- R315A + L663N + I595M
- T49D + P124D + I595M (QES.i01)
- P124D + L663N
- T49D + R315A + I595M + K117Y
- T49D + R315A + I595M + R166L
- T49D + R315A + I595M + L663N
- T49D + K117V + R315A
- K117V + R315A + L663N
- K117V + R166L + F223Y + I595M
- K117V + R166L + F223Y + L663N
- P124D + R315A
- P124D + R315A + L663N
- T49D + R315A + I595M + L663N
- T49D + P124D + R315A + I595M + L663N
- T49D + P124D + R315A + I595M
- T49D + P124D + I595M + L663N
- T49D + P124D + R315A + G514P + I595M
- T49D + P124D + L663N (QES.i02)
- I181L + V254T (QES.c02)
- I181L + V255M
- V254T + V255M
- T49D + P124D + I595M + I181L
- T49D + P124D + I595M + V254T
- T49D + P124D + I595M + I181L + V255M
- (QES.i01.c01)
- V181L + V254T
- V181L + V255M
- V181L + V254T + V255M (QES.c03)
- P124D + I595M
- P124D + F223Y + I595M
- A200E + F223Y + I595M (QES.i03)
- P124D + R557Q
- R557Q + F223Y
- P124D + A200E + F223Y + I595M
- A200E + F223Y + R557Q + I595M
- R166L + A200E
- R166L + R557Q
- R166L + R557Q + I595M
- A200E + R557Q + I595M
- P124D + A200E
- P124D + R166L
- P124D + R557Q + I595M
- P124D + R166L + R557Q
- R166L + A200E + R557Q
- R166L + F223Y + R557Q
- P124D + R166L + R557Q + I595M
- P124D + F223Y + R557Q + I595M (QES.i04)
- I181L + V254T + V255M
- P124D + F223Y + R557Q + I595M + I181L
- (QES.i04.I181L)
- P124D + F223Y + R557Q + I595M +
- I181L + V255M
- K117Y + A200E
- T163D + A200E
- A200E + L581D
- A200E + R557Q
- A200E + I595M
- V181L + A200E
- K117Y + L581D
- A200E + F223Y + R557Q
- A200E + L581D + I595M
- A200E + L581D + I595M + L663N
- K117Y + L581D + I595M
- K117Y + L581D + I595M + L663N
- A200E + F223Y + I595M + V181L;
- and
- A200E + F223Y + I595M + V181L + V255M
- (QES.i03.c01)
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Dependencies are inferred from the claim text.