Novel dnase
AssigneeKatholieke Universiteit Leuven
InventorsAbram Aertsen, Hanne Hendrix, Rob Lavigne, Marie Van Der Gucht
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Abstract
The present invention relates to a group of proteins from bacterial phages which show DNA degrading activity.
Claims28
-32. (canceled)
An in vitro method of degrading DNA, the method comprising:
- contacting a DNA comprising sample with a polypeptide comprising a sequence with at least 60% identity with any one of the sequences of SEQ ID NO:
- 1 to SEQ ID NO:
- 9 or a fragment thereof, wherein the polypeptide or fragment thereof has nuclease activity.
A pharmaceutical composition comprising a polypeptide comprising a sequence with at least 60% identity with any one of the sequences of SEQ ID NO:
- 1 to SEQ ID NO:
- 9 or a fragment thereof, wherein the polypeptide or fragment thereof has nuclease activity.
A method of treating cystic fibrosis, the method comprising administering an effective amount of a polypeptide comprising a sequence with at least 60% identity with any one of the sequences of SEQ ID NO:
- 1 to SEQ ID NO:
- 9 or a fragment thereof, to an animal or human in need thereof, wherein the polypeptide or fragment thereof has nuclease activity.
A method of treating a bacterial infection, the method comprising administering an effective amount of a polypeptide comprising a sequence with at least 60% identity with any one of the sequences of SEQ ID NO:
- 1 to SEQ ID NO:
- 9 or a fragment thereof, to an animal or human in need thereof, wherein the polypeptide or fragment thereof has nuclease activity.
A polypeptide comprising a sequence with at least 60% identity with any one of the sequences of SEQ ID NO:
- 1 to SEQ ID NO:
- 9 or a fragment thereof, wherein the polypeptide or fragment thereof has nuclease activity, with the proviso that the polypeptide is not a polypeptide consisting of a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8 and SEQ ID NO:
- 9.
An antibody specifically binding to a polypeptide comprising a sequence with at least 60% identity with any one of the sequences of SEQ ID NO:
- 1 to SEQ ID NO:
- 9 or a fragment thereof, wherein the polypeptide or fragment thereof has nuclease activity.
A method of identifying modified versions of a polypeptide selected from the group consisting of SEQ NO:
- 1 to 9:
- with nuclease activity, the method comprising:
- introducing one or more changes in the nucleotide sequence of a polynucleotide encoding a sequence selected from the group consisting of SEQ NO:
- 1 to 9, the one or more changes resulting in a modified amino acid sequence;
- expressing the protein with the modified amino acid sequence;
- and
- assaying the expressed protein for nuclease activity.
Dependencies are inferred from the claim text.