Double-layer dressing containing silk fibroin and a method for making the same
AssigneesNational Taiwan University of Science and Technology, Life Star International Limited
InventorsMENG-YI BAI, MENG-CHUAN CHEN, JIA-YING LIN, WEI-YIN CHEN
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Abstract
Disclosures of the present invention describe a double-layer dressing containing silk fibroin and a method for making the same. The double-layer dressing mainly comprises a silk fibroin layer and a calcium-degradation silk fibroin layer connected to the silk fibroin layer. It is worth emphasizing that, results of animal experiment have proved that this novel double-layer dressing is an outstanding hemostatic wound dressing. Moreover, additional adhesion, resulted from the solidification of tissue fluid, can be effectively prevented from forming between skin wound and wound dressing under the use of this double-layer dressing.
Claims14
A double-layer dressing containing silk fibroin, comprising:
- a silk fibroin layer;
- and
- a calcium-degradation silk fibroin layer, being disposed on the silk fibroin layer.
A method for making double-layer dressing containing silk fibroin, comprising following steps:
- (1) preparing a raw material of silkworm cocoon, and then producing a silk fibroin product by applying a degumming process to the raw material of silkworm cocoon;
- (2) dissolving the silk fibroin product in a salt solution, so as to obtained a silk fibroin solution;
- (3) applying a dialysis process to the silk fibroin solution, thereby obtaining a dialysate of silk fibroin;
- (4) separating the dialysate of silk fibroin into a first dialysate and a second dialysate;
- (5) producing a first supernatant by applying a first centrifuging process to the first dialysate;
- (6) applying a degradation process to the second dialysate so as to obtain a protein-degraded solution, and subsequently producing a mixture solution by mixing the protein-degraded solution with a solution of bivalent metal salt;
- (7) producing a second supernatant by applying a second centrifuging process to the mixture solution;
- (8) applying a freeze-drying process to both the first supernatant and the second supernatant thereby respectively obtaining a silk fibroin layer and a calcium-degradation silk fibroin layer, and then producing a double-layer dressing containing silk fibroin by disposing the calcium-degradation silk fibroin layer on the silk fibroin layer.
Dependencies are inferred from the claim text.