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US 2020/0172889 A1Published application
Protease and Binding Polypeptide for O-Glycoproteins
AssigneeGenovis AB
InventorsFredrik Leo, Rolf Lood, Stephan Bjork, Malin Mejare, Fredrik Olsson
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Abstract
The present invention relates to a novel endoprotease, mutants thereof having binding but lacking or having reduced hydrolyzing activity, and use in methods of studying and isolating O-linked glycoproteins.
Claims20
A polypeptide having endoprotease activity specific for O-glycosylated proteins which comprises:
- (a) an amino acid sequence of SEQ ID NO:
- 1;
- (b) an amino acid sequence which is at least 85% identical to the amino acid sequence of SEQ ID NO:
- 1;
- or
- (c) an amino acid sequence which is a fragment of the sequence of SEQ ID NO:
- 1 or a fragment of an amino acid sequence which is 85% identical to the amino acid sequence of SEQ ID NO:
- 1.
5 more elements
A polypeptide which is capable of binding to an O-glycan or O-glycoprotein and which lacks or has reduced endoprotease activity specific for O-glycosylated proteins comprising:
- (a) an amino acid sequence of SEQ ID NO:
- 5 or SEQ ID NO:
- 20;
- (b) an amino acid sequence which is at least 85% identical to the amino acid sequence of SEQ ID NO:
- 5 or SEQ ID NO:
- 20;
- or
- (c) an amino acid sequence which is a fragment of the sequence of SEQ ID NO:
- 5 or SEQ ID NO:
- 20 or a fragment of an amino acid which is 85% identical to the amino acid sequence of SEQ ID NO:
- 5 or SEQ ID NO:20.
8 more elements
Dependencies are inferred from the claim text.